inclusion bodies, purification of
un691cs at genius.embnet.dkfz-heidelberg.de
Tue Dec 3 05:55:54 EST 1996
In article <32A2F2D4.4E89 at snoopy.org.chemie.tu-muenchen.de>,
schott at snoopy.org.chemie.tu-muenchen.de says...
>Does anybody know a simple method, (or a literature citation) for
>separating inclusion bodies from bacterial debris? (probably by
Try to get a protocol booklet from either Novagen or Qiagen:
they describe methods how to purify His-Tagged proteins from
In principal, I spin the E. colis at 5 K, then dissolve the
pellet in PBS, Then give them 5 * 10 seconds on ice-water (!)
at fullblast setting of ultrasound. Then spin again at 5k.
The pellet now contains bacterial debris.
Spinning at 13k results in a white pellet of inclusion
bodies that then can be opened with high Urea (6M).
More information about the Methods