Freeze-thawing of primers

slavemaster un691cs at
Tue Dec 3 05:49:41 EST 1996

In article <dmachugh-0212961254100001 at>, dmachugh at says...

... snip-a-dee-doo-da-snip-a-dee-do...

>However, in the last week or two the yield has dropped off dramatically
>and a new primer-artifact has appeared of approximately 80-90bp.
....> Obviously, this artifact is reducing the yield of the target by
>competing for primer.  I have a number of ideas as to why this may be
>happening, but the most likely reason in my opinion may be due to
>freeze-thawing degradation of the primers resulting in the appearance of
>this primer artifact.  

yes, we had this problem. It went away when we made aliquots of our primers
and stored these at -20C.


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