Nde I restriction enzyme

Robert Cowherd rcowherd at mail.med.upenn.edu
Tue Dec 3 10:21:54 EST 1996

A year or two ago I was routinely digesting an internal PCR control fragment
(mimic) with Bcl I (I know, not the same as Nde I) right in the post run PCR 
reaction itself.  Worked "like a charm."  Can you possibly do the same?  
How about some modification of the PCR reaction itself that would obviate 
phenol-chloroform extraction and ETOH precipitation?

Just my $0.02 worth...

Bob Cowherd

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