Nde I restriction enzyme
rcowherd at mail.med.upenn.edu
Tue Dec 3 10:21:54 EST 1996
A year or two ago I was routinely digesting an internal PCR control fragment
(mimic) with Bcl I (I know, not the same as Nde I) right in the post run PCR
reaction itself. Worked "like a charm." Can you possibly do the same?
How about some modification of the PCR reaction itself that would obviate
phenol-chloroform extraction and ETOH precipitation?
Just my $0.02 worth...
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