Nitrocellulose membrane and UV cross-linking.

Yoonsuk Park yoonpark at u.washington.edu
Wed Dec 4 14:03:25 EST 1996


Hi, everyone,

I'm trying to colony hybridization using nitrocellulose membrane (S&S).
According to the manual which the manufacturer provided, vacuum drying oven
should be used to fix DNA onto nitrocellulose, but UV cross-linker can be
used for nylon membrane.  Is it true that UV cross-linker cannot be used
for nitrocellulose membrane for this purpose? If it's, why?

If someone knows about this, can you explain it to me?
Thanks in advance!

YP.

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Yoonsuk Park, Ph.D.                 
University of Washington                 e-mail) yoonpark at u.washington.edu
Dept. of Oral Biology, Box 357132        Phone)  206-543-5477 (ext. 40)       
Seattle, WA, 98195-7132                  Fax)    206-685-3162
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