run-off translation in vitro?

Scott Vande Pol sbv at pop.cwru.edu
Wed Dec 4 13:42:10 EST 1996


I would like to make a series of 3' deletions in proteins that are
translated in vitro by cutting within the gene with restriction enzymes and
in vitro transcribing and translating the resulting plasmids.  
1.  Will ribosomes in vitro translate such products efficiently where
termination is not due to a stop codon but rather due to an end in the
mRNA?
2.  Will they translate to the end of the message or truncate a few codons
before the end?

Any experience of this?

-- 
Scott Vande Pol
Case Western Reserve University
Department of Pathology
Cleveland Oh. 44106
sbv at pop.cwru.edu



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