Lysis of bacteria in maxi prep.

Florence CABON cabon at infobiogen.fr
Wed Dec 4 07:09:23 EST 1996


Dear netters,

I had problems recently with a qiagen maxi prep. I was preparing two
plasmids. The first prep went OK but I failed to lyse the second one. I
tried to add some SDS, I added some lysozyme, I warmed the mix. The
bacteria were still intact in suspension. Two days later we started the
prep again, draining carefully all the LB medium from the bacterial
pellet. The result was the same.
The plasmid is in a classical puc backbone. The bacteria are Xl1blue
from stratagene
Any advise ?

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Florence Cabon. CNRS Villejuif, France. cabon at infobiogen.fr
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