DNA yield from plant tissue.
J. Pat Martinez
martinez at puccini.CRL.umn.edu
Wed Dec 4 15:34:22 EST 1996
What type of detergent did you use in your extraction? If you used CTAB,
then that can account for the artificially high OD readings.
<Pine.OSF.3.91.961204145039.4932A-100000 at eduserv.its.unimelb.EDU.AU>,
Michael Adamson <s_mka1 at eduserv.its.unimelb.EDU.AU> wrote:
> Does anyone know the theoretical upper limit for genomic DNA/mg in
> expanding eucalyptus leaf tissue? I'm a bit worried that my 2ug/mg yield
> from leaf discs is suspiciously high. Even commercial kits advertise only
> a 0.3-0.8ug/mg DNA yield. The pellets are also a pink-purple colour, but
> 260/280 is always 1.8-1.9. Could RNA be the culprit (though there's only a
> fairly faint smear at the bottom of my gels)? I don't use RNase, but I
> would have thought that not using RNA-grade reagents or equipment should
> have resulted in much of it degrading - especially considering the 40min
> incubation in non-sterilised lysis buffer.
> Any ideas greatly appreciated!
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