PGal concentration

André Gallant soniag at nbnet.nb.ca
Wed Dec 4 19:35:56 EST 1996


Hi everyone,

I've been using phenyl-B-D-galactoside (PGal) to select E. coli lacI
mutants, but haven't have too much success (even tried wt with IPTG). 
Is it possible that my PGal concentration is too high (750 mg/L)?  Does
anyone know the optimal concentration of (PGal) to select lacI mutants.

Thanks....in advance!
-- 
Andre Gallant
Grad student
Dept Chimie-Biochimie
Universite de Moncton
E-mail:  soniag at nbnet.nb.ca

"If I knew what I was looking for...It wouldn't be called research"
.



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