Nitrocellulose membrane and UV cross-linking.

Kathleen Schroeder kes5 at mail.duke.edu
Wed Dec 4 19:03:42 EST 1996


I used to do UV crosslinking for plaque lifts on nitrocellulose all the time 
and had good luck. 

In article 
<Pine.A41.3.95b.961204105651.154004A-100000 at homer35.u.washington.edu>, 
yoonpark at u.washington.edu says...
>
>Hi, everyone,
>
>I'm trying to colony hybridization using nitrocellulose membrane (S&S).
>According to the manual which the manufacturer provided, vacuum drying oven
>should be used to fix DNA onto nitrocellulose, but UV cross-linker can be
>used for nylon membrane.  Is it true that UV cross-linker cannot be used
>for nitrocellulose membrane for this purpose? If it's, why?
>
>If someone knows about this, can you explain it to me?
>Thanks in advance!
>
>YP.
>
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
>Yoonsuk Park, Ph.D.                 
>University of Washington                 e-mail) yoonpark at u.washington.edu
>Dept. of Oral Biology, Box 357132        Phone)  206-543-5477 (ext. 40)       
>Seattle, WA, 98195-7132                  Fax)    206-685-3162
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