Rescuing plasmid from dead E.coli stock.

Emma Macfarlane emma at HANCOCK.MICROBIOLOGY.UBC.CA
Thu Dec 5 13:00:57 EST 1996


I remember reading some time ago on this newsgroup about retreiving 
plasmid from dead plates to re-transform into E.coli, but I can't
find reference to it in the archives. Is it possible to
do the same thing with frozen stocks? We have an important construct 
in a sickly E.coli strain that is now refusing to grow. I tried
taking a scraping of cells, boiling it up in water, spinning it down
and using the supernatant to transform DH5a. This didn't work.
If anyone has an alternative suggestion I would appreciate hearing it.
Please reply direct to my email address emma at hancock.microbiology.ubc.ca 
as I am not a regular subscriber to the newsgroup.
Thanks in advance
Emma




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