Insertional inactivation of genes

Farid Jalali fjalali at
Wed Dec 4 23:40:35 EST 1996

Hello All,

I would like input regarding how I might insertionally inactivate or
delete a region of genomic DNA, from a cyanobacterium, that exists in an
operon. The antibiotic resistance cassettes I am using now, have within
them transcriptional and translational stop sequences. I want to disrupt
upstream sequences without disrupting the expression of downstream

Any advice or input would be greatly appreciated.


Farid Jalali
Department of Botany
Erindale College
University of Toronto

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