Biotin/streptavidin/blocking

Mike Garbutt mgarbutt at gpu.srv.ualberta.ca
Thu Dec 5 01:32:20 EST 1996


Fellow researchers:
I've boitinylated cultured cells, lysed with 2X SDS gel loading buffer with 
BME, sheared DNA, ran on SDS PAGE, transfered to PVDF, blocked with 5% 
milk/TBS+0.05% tween 20, probed with streptavidin-HRP conjugate and 
detected with ECL detection solutions 1+2, home made but good:-)
I would like to know what is the best blocking solution for this procedure?
Thanks, Mike
-- 
Mike Garbutt,
Dept. of Anatomy and Cell Biology,
6-32B Medical Science Building,
University of Alberta,
Edmonton, AB.
Canada.T6G 2M7
Tel. (403) 492 6485 (Work)
Tel. (403) 465 9285 (Residence)
e-mail:   mgarbutt at gpu.srv.ualberta.ca
homepage: http://www.ualberta.ca/~mgarbutt/



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