maieru at uni-muenster.de
Thu Dec 5 06:00:36 EST 1996
a03m at biologie.uni-bremen.de (Georg Kroeger) wrote:
>we found an old box with actinomycin D in our fridge. We even got some
>bacteria, but how should we put them together. Could anyone be so kind
>and give us informations about how to dissolve it, and about useful
>concentrations for procaryotes!
>Thanks in advance!!
You can dissolve Actinomycin D in pure Ethanol in a concentration of
5mg/ml as a stock solution.
I have used it for human fibroblasts with a final concentration of
5µg/ml medium, but you´ll have to check the optimal concentration for
your assay by measuring the incorporation of 3H-uridine in the total
RNA after a short-time incubation of the bacteria with the
Actinomycin D blocks between 95 and 99% of overall transcription.
Yours, U. Maier
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