Nitrocellulose membrane and UV cross-linking.

Hiranya Roychowdhury hroychow at NMSU.EDU
Thu Dec 5 11:42:29 EST 1996


At 11:03 AM 12/4/96 -0800, Yoonsuk Park wrote:
>Hi, everyone,
>
>I'm trying to colony hybridization using nitrocellulose membrane (S&S).
>According to the manual which the manufacturer provided, vacuum drying oven
>should be used to fix DNA onto nitrocellulose, but UV cross-linker can be
>used for nylon membrane.  Is it true that UV cross-linker cannot be used
>for nitrocellulose membrane for this purpose? If it's, why?

because NC goes *poof* when exposed to UV.

>
>If someone knows about this, can you explain it to me?
>Thanks in advance!
>
>YP.
>
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
>Yoonsuk Park, Ph.D.                 
>University of Washington                 e-mail) yoonpark at u.washington.edu
>Dept. of Oral Biology, Box 357132        Phone)  206-543-5477 (ext. 40)       
>Seattle, WA, 98195-7132                  Fax)    206-685-3162
><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
>
>
>

Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu



More information about the Methods mailing list