Automatic sequence analyzer???
ahnss at hitel.kol.co.kr
Thu Dec 5 07:30:50 EST 1996
I am performing sequencing by automatic sequence analyzer(ALFexpress,
Pharmarcia) for 1.1 kb gene (GC rich, about 70%) located in nearby
At first trial using universal primer it read through the sequence about
660 bp in length, but from site of 350 bp some errors were shown. so, i
made a 19mer primer at the site about 300 bps for directional sequencing.
In this time, there are no peaks except only the primer. The operator won't
read the sequence. At first, i just doubt the purity the plasmid sample, so
i used colume for the plasmid DNA purity, desperately, the result was the
I used the template plasmid in 5-10 micrograms and 100 pmol of the primer.
Any help or suggestion would be greatly aprepriated.
E. mail ad. ahnss at hitel.kol.co.kr
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