Gel purification of YAC DNA

Veera Padmanabhan v0p8630 at ACS.TAMU.EDU
Fri Dec 6 01:59:10 EST 1996


Dear Netters,

I am having difficulty in isolating megabase DNA (YAC DNA) from the TBE 
gels for making whole YAC probes and also for random subcloning. Could 
anyone suggest me which one is the best method such as GEN CLEAN, phenol 
extraction, agarase digestion etc.,

Thank You,
Veera



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