DNA yield from plant tissue.
Thu Dec 5 20:07:39 EST 1996
I have tried extracting both RNA and DNA from cycads. Using a hot phenol
method, I got something red co-precipitating with my DNA and RNA pellets.
The OD readings seemed normal, but the yield looked low when run on a gel,
and they were poor RT-PCR and PCR templates. Using CTAB actually helped
me by removing the red contaminant, which was presumably some sort of
secondary metabolite in cycads.
banoo at bio.uottawa.ca
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