Rules of thumb for messing with vector's LacZ' coding seq?

Mike Prigge prigge at darkwing.uoregon.edu
Fri Dec 6 02:59:36 EST 1996


I would like to add a pair of SfiI sites to a vector containing the alpha
complementation peptide. I could drop in a 27 bp oligo, ie, a 9 aa
insertion. Should this, most likely, be okay? (I am guessing no since
cloning small PCR products have given me blue colonies before.) Are there
any particular amino acids that should be avoided within this fragment?
Thanks
Mike
Michael J. Prigge                     prigge at darkwing.uoregon.edu
University of Oregon               Institute of Molecular Biology
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