pUC21 sequence? (or..."pSK"= "pBluescript SK")

Mike Prigge prigge at darkwing.uoregon.edu
Fri Dec 6 02:51:45 EST 1996


I was wondering if anyone had the sequences for any of the pUC21 generation 
vectors (pUC21, pUK21, & pOK21; Viera & Messing, 1991. Gene 100:189-94)?
VectorDB has only the MCS. Although they are essentially pUC118 w/ a new
MCS, a T7 site is also added from "pSK from Stratagene." Would this be the
same as pBNluescript SK? If so, I am curious as the sequence between the
right SpeI site and the T7 region. Has anyone sequenced acrossed it with a
M13 primer?

Thanks, 
Mike
Michael J. Prigge                     prigge at darkwing.uoregon.edu
University of Oregon               Institute of Molecular Biology
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