Blot with TEFLON membrane, references?

[Armin Engels]

>+>>I understand that PVDF membrane blot cannot be used in HP 
>C-terminal
>+>>sequencing procedures. Instead, Teflon membrane blot has to be 
>used. Does
>+>>anyone have a 
>+>>reference for Teflon membrane blotting?

>+>see:
>+>Burkhart,WA et al.(1996), ANALYTICAL BIOCHEMISTRY 236, pp 364-367
>+>

>Am I wrong in thinking that Teflon is just a proprietry name for PVDF?
>..d
>* David Martin - Atherosclerosis and Thrombosis research group  *
>* http://www.uio.no/~damartin/      david.martin at biotek.uio.no  *
>* Arbeid. 22 95 84 54 Telefax. 22 69 41 30  Mobil. 90 74 27 65  *
>

Hi David,

Teflon and PVFD are different polymers:

Teflon ...-CF(2)-CF(2)-...
PVDF   ...-CH(2)CF(2)-CH(2)CF(2)- ....

Armin