What makes gels smile?

Matt thomas Thomas at molbio.uoregon.edu
Fri Dec 6 20:55:58 EST 1996


Anyone have any ideas on how to prevent gels from smileling?  In 
particular I'm talking about Urea/acrylamide gels, the kind typically 
used for sequenceing.  I've heard all kinds of folk lore on this but 
I'm interested in if anyone has a sure fire way to keep the smile from 
happening.  

I've tried running fast or slow, long or short prerunning times, long 
or short polymerization times, but I haven't seen a real correlation 
between all these.  

Any suggestions would be helpfull.

Thanks,
Matt thomas
Thomas at molbio.uoregon.edu



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