Cocktail sticks and toothpicks supplier
102063.2640 at CompuServe.COM
Sun Dec 8 21:52:16 EST 1996
The technique I've always used instead of toothpicks is the real
inexpensive pointed or circular plastic loops that many suppliers
(such as Elkay, american Scientific, etc.) sell; you pick the colony
with the tip of the loop, touch the medium in a sterile tube with it
(so that you can grow an overnight culture if the PCR result
warrants it), then touch the tip into ~50 ul of water in a 1.5ml
microfuge tube, and cut off the end of the loop so that it falls
into the tube. Cap the tube, boil for 10min, spin, and you've got
PCR-ready DNA. This also works well for phage plaques.
-- Bob O.
(No unethical affiliation with any of these companies)
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