What factors effect protein migration on SDS-PAGE
David.Hills at bbsrc.ac.uk
Mon Dec 9 05:55:52 EST 1996
Some other factors are:
Do you have a very basic protein..pI near 10...lots of lysine. Histones
can migrate anomalously. Affected by phosphorylation??
Also is it just the protein? Do you have upstream AUGs in the message..or
even CUG or AUU!! I have seen expression from an AUU codon in one of the
baculovirus trabfer vectors many moons ago....pVL1392 was it? Check these
out and also the initiation context of your AUG, which may not be
favouravle if there are these others around.
Hope this is a VERY small but not insignificant amount of help!
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