purification of His-tagged proteins

[Volker Blaschke]

Who has managed to perform a purification of a His-tagged protein from 
mammalien cells ( in this case HEK 293 cells ) with a nickel -chelate 
column ?
Was it a special kit that you have taken ?
Which detergens have you used to solubilize the protein ? (in case of a
membrane-standing protein )
I would be delighted to get some information about it !
My E-mail:  uboeer at gwdg.de