nick Translation

Krish Roy kroy at
Sat Dec 7 16:13:05 EST 1996

I am trying to radio-label a plasmid.  my question is after the nick
translation procedure using the plasmid does the labeled DNA stay in
plasmid form...I absolutely need it to stay as a circular plasmid. I ran
a gel after passing through a sephadex column and the gel was a all oligonucleotides...why should that happen!!!! Please
help..if anyone knows how to label a circular plasmid please let me
please reply to kroy at

Thanka a lot..
krish Roy
Johns hopkins

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