nick Translation

Krish Roy kroy at bme.jhu.edu
Sat Dec 7 16:13:05 EST 1996


Hi..
I am trying to radio-label a plasmid.  my question is after the nick
translation procedure using the plasmid does the labeled DNA stay in
plasmid form...I absolutely need it to stay as a circular plasmid. I ran
a gel after passing through a sephadex column and the gel was a
smear..like all oligonucleotides...why should that happen!!!! Please
help..if anyone knows how to label a circular plasmid please let me
know..
please reply to kroy at bme.jhu.edu

Thanka a lot..
krish Roy
Johns hopkins



More information about the Methods mailing list