Protein sequencing ??!!

[jstrassw at OPAL.TUFTS.EDU]

Hello!
The rule of thumb seems to be that if you have a fainly Coomassie 
stainable band on your gel, then you can have n-terminal 
microsequencing.  I cant imagine why a yeast person yould resoprt to such 
an approach.  There are som many interesting genetic screens one can 
employ in S. cerivissiae.

John


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John Strasswimmer, MD,PhD Candidate    | Phone (617) 636 8396
Tufts - New England Medical Center     | Fax   (617) 636 6190
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     *** Co-Author of "HyperBug" Microbiology Teaching Software ***
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On Mon, 9 Dec 1996, Adrian Bracken wrote:

> I plan to run total yeast proteins on SDS gels. Certain lanes will contain
> bans not present in other lanes....
>    I am wondering if anybody out there has knowledge about cutting out and
> sequencing such proteins? I would be very grateful if you could reply to
> brackena at tcd.ie. Thanks very much, Adrian Bracken.
> 
>