Microwave sterilization

Mike Prigge prigge at darkwing.uoregon.edu
Mon Dec 9 14:48:49 EST 1996


In article <58hcnp$149 at is.bbsrc.ac.uk>, David Hills
<David.Hills at bbsrc.ac.uk> wrote:

> Usual situation: gone to cupboard to get sterile bottle and the last one 
> has been used (what else should you expect!). Can a microwave be used to 
> sterilise plastics/glassware as a last resort? I have just discovered 
> that 5 mins Full power (600W) seems to bend large polystyrene dishes, 
> although a couple of 1L bottles on top straighten them out!
> 
> Anybody out there use this method of sterilization??
> Dave
> 
I have done basically the same thing. I have sterilized Arabidopsis seed
(for plating) by putting them in a polypropylene 15ml tube and nuking for
2 X 5 minutes. Evidently, this is hard on microwaves, so put 100 ml of H2O
with boiling stones in a beaker in there too.
Mike
Michael J. Prigge                     prigge at darkwing.uoregon.edu
University of Oregon               Institute of Molecular Biology
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