trimming polyA tails

Graeme Wistow graeme at mge2.nei.nih.gov
Mon Dec 9 14:01:02 EST 1996


 Hi,

 I'm looking for a reference and/or protocol for improving the
 resolution of northerns by removing the polyA tail from mRNA.
 
 I've seen this somewhere but I can't trace it. I think it
 involves use of oligo(dT) and RNaseH. I recall seeing blots 
 with sharp bands instead of the smudge resulting from 
 variable tail length.
 
 Does this ring any bells?
 
 
 Graeme Wistow, Ph.D.
 6/222, NIH,
 Bethesda, MD 20892-2730 USA
 
 301-402-3452
 301-496-0078 (fax)
 graeme at mge2.nei.nih.gov



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