blunt ligation

Hiranya Roychowdhury hroychow at NMSU.EDU
Mon Dec 9 12:52:32 EST 1996

At 04:44 PM 12/8/96 -0800, Chang,jihun wrote:
>Dear bionetter.
>I'm trying to ligate Hind III-digested genomic DNA into SmaI-digested 
>pBluescript SK vector.
>but, final white transformants I got were so few.
>I want many white colonies.
>Please, give me your help.

How on earth do you expect to ligate this with any efficiency?

Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at

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