denaturing DNA in 0.12Mpb

Andrea Crampton s316741 at student.uq.edu.au
Tue Dec 10 11:20:37 EST 1996


HI
	I am having trouble denaturing 40ug of DNA, it is resuspended in 0.12M
phosphate buffer (this i can not change) after 10 minutes in boiling
water there is still a lot of DNA that has not denatured.

So any ideas>>>>>>>>>I need at least 95% denaturation.....

Thanks



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