Req: Best transformation protocol for LCL's

* * at alf1.ngate.uni-regensburg.de
Mon Dec 9 07:42:08 EST 1996


Hi

LCLs seem to show a very poor transformation ratio.
So I am curious to hear of any suggestions from you out there to
improve this. 
How about Lipofectamine compared to electroporation?
How low can you go with your DNA-concentrations? 
Any electroporation profiles that work best?
Does DMSO increase efficiency?

looking forward for suggestions

Mike





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