Req: Best transformation protocol for LCL's
*
* at alf1.ngate.uni-regensburg.de
Mon Dec 9 07:42:08 EST 1996
Hi
LCLs seem to show a very poor transformation ratio.
So I am curious to hear of any suggestions from you out there to
improve this.
How about Lipofectamine compared to electroporation?
How low can you go with your DNA-concentrations?
Any electroporation profiles that work best?
Does DMSO increase efficiency?
looking forward for suggestions
Mike
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