Automatic sequence analyzer???

Dr. Duncan Clark duncan at genesys.demon.co.uk
Mon Dec 9 05:39:21 EST 1996


In article <01bbe2a8$aa17f320$c091fccc at ------>, Takbum Ohn
<ahnss at hitel.kol.co.kr> writes
>I used the template plasmid in 5-10 micrograms and 100 pmol of the primer.

That seems very very high. I would have thought 0.5ug and a couple of
pmol primer would be ample assuming you are doing cycle sequencing.

Duncan 
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Dr. Duncan Clark
DNAmp Ltd.
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