Automatic sequence analyzer???

Dr. Duncan Clark duncan at
Mon Dec 9 05:39:21 EST 1996

In article <01bbe2a8$aa17f320$c091fccc at ------>, Takbum Ohn
<ahnss at> writes
>I used the template plasmid in 5-10 micrograms and 100 pmol of the primer.

That seems very very high. I would have thought 0.5ug and a couple of
pmol primer would be ample assuming you are doing cycle sequencing.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Dr. Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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