Protein Purification Help

[Kurt L Brillhart]

Simple-  just equilibrate your desalting column with the buffer that you want 
your enzyme to end up in.  Most of these columns have a MW cutoff of about 
5000, so glycerol won't be separated out.  Keep in mind that sample size makes 
a big difference in the results, however.  If you push the limit of column 
capacity (about 25% of gel volume for Sephadex G25) you remove only about 90% 
of the low MW material.

Kurt Brillhart