denaturing DNA in 0.12Mpb

[Hiranya Roychowdhury]

At 04:20 PM 12/10/96 +0000, Andrea Crampton wrote:
>HI
>	I am having trouble denaturing 40ug of DNA, it is resuspended in 0.12M
>phosphate buffer (this i can not change) after 10 minutes in boiling
>water there is still a lot of DNA that has not denatured.
>
>So any ideas>>>>>>>>>I need at least 95% denaturation.....
>
>Thanks
>
>

IMO, 120mM salt is a lot. Try reducing the salt if you want complete denaturation of the DNA.


Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu