Protein sequencing ??!!

Adrian Bracken brackena at tcd.ie
Tue Dec 10 08:29:59 EST 1996


Can you send me on a protocol? 

In article <Pine.PMDF.3.91.961209183717.111142B-100000 at OPAL.TUFTS.EDU>,
jstrassw at OPAL.TUFTS.EDU wrote:

> Hello!
> The rule of thumb seems to be that if you have a fainly Coomassie 
> stainable band on your gel, then you can have n-terminal 
> microsequencing.  I cant imagine why a yeast person yould resoprt to such 
> an approach.  There are som many interesting genetic screens one can 
> employ in S. cerivissiae.
> 
> John
> 
> 
> ------------------------------------------------------------------------
> John Strasswimmer, MD,PhD Candidate    | Phone (617) 636 8396
> Tufts - New England Medical Center     | Fax   (617) 636 6190
> Box 166                                | Email:  jstrassw at opal.tufts.edu
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> ------------------------------------------------------------------------
> 
> On Mon, 9 Dec 1996, Adrian Bracken wrote:
> 
> > I plan to run total yeast proteins on SDS gels. Certain lanes will contain
> > bans not present in other lanes....
> >    I am wondering if anybody out there has knowledge about cutting out and
> > sequencing such proteins? I would be very grateful if you could reply to
> > brackena at tcd.ie. Thanks very much, Adrian Bracken.
> > 
> >



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