Sabine Hanelt sabine.hanelt at medizin.uni-ulm.de
Tue Dec 10 08:12:46 EST 1996

Hi netters,
in our group we are working with the hprt mutation assay and the BrdU assay to 
detect HPRT deficient clones.
Since some weeks we have a never ending discussion about two things. 
The first is: what does 6-TG (thioguanine) exactly do so that hprt proficient 
cells die. Does the polymerization stop or are the enzymes not able to 
translate mRNA with 6-TG into functional proteins, or both of it.
The second is: for the BrdU assay we use 6-TG in much higher concentrations 
(34 µg/ml) than used in the "classical" hprt assay (2-10 µg/ml). Does 6-TG 
bloc the growth of the cells when it is used in higher concentrations and if 
this is the case, in which form does it bloc.
Thank's for your help

More information about the Methods mailing list