trimming polyA tails

SCLAB jzhang at
Tue Dec 10 03:09:00 EST 1996

There's an older paper - PNAS (1975) 72(8):2959-63 - dealing with this.

I believe others have used a specific oligo when the sequence near the
3' end of the transcript was known well enough to design one, and it
was supposedly cleaner after RNase H digestion than oligo dT.  May have 
even been in one of the Clontechniques, or Focus type publications a few 
years back, but not sure which.


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