SDS-PAGE SAMPLES

KAREN MJFASKH at FS1.CE.UMIST.AC.UK
Tue Dec 10 20:54:06 EST 1996


I'm trying to run protein samples which contain 6 M guanidine HCl on SDS
PAGE gels, however when I add the sample buffer the sample precipitates
and if I try to load it on the gel I don't get anything useful back! Can
anyone suggest a way to get these samples on a gel short of actually
dialysing them first?

Alternatively, is there any way of dialysing lots of small samples
fairly easily without the protein sticking to the membrane?

Any ideas?

Karen



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