How to remove RNase from protein extract?

nhwu at PUBLIC3.BTA.NET.CN nhwu at PUBLIC3.BTA.NET.CN
Tue Dec 10 22:30:39 EST 1996


Hello there:

I am doing experiments on a kind of RNA binding protein. Unfortunately, I 
found the RNAs I had synthesized were all degraded by the RNase exsits in 
the Protein extract. I used  to add RNasin in the reaction chamber,but it 
did not work. Can you tell me how to discard the RNase without interfering 
with the acitvities of other proteins? 

Any suggestion will be greatly appreciated!

Toy



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