tbelbin at morgan.ucs.mun.ca
Tue Dec 10 18:55:51 EST 1996
In article <32AE143E.3636 at FS1.CE.UMIST.AC.UK>, MJFASKH at FS1.CE.UMIST.AC.UK wrote:
> I'm trying to run protein samples which contain 6 M guanidine HCl on SDS
> PAGE gels, however when I add the sample buffer the sample precipitates
> and if I try to load it on the gel I don't get anything useful back! Can
> anyone suggest a way to get these samples on a gel short of actually
> dialysing them first?
> Alternatively, is there any way of dialysing lots of small samples
> fairly easily without the protein sticking to the membrane?
> Any ideas?
Guanidine will ppt in the presence of SDS. If you do not want to
dialyze your samples, you might try switching to a buffer containing
8M urea during the procedure (eg. column washing).
Only other thing to do would be to TCA ppt your samples and resuspend them
in the buffer of your choice.
Hope this helps...
Biochemistry Department, Memorial University of Newfoundland
St. John's, NF Canada A1B 3X9
Phone: (709)-737-2538 Fax: (709)-737-2422
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