cycle sequencing
Els Van Geldre
Els.VanGeldre at rug.ac.be
Wed Dec 11 11:39:57 EST 1996
Hi,
I'm wondering wether it is possible to perform cycle sequencing reactions
directly on genomic DNA with a specific primer. I would like to use this
technique in order to isolate a gene of about 2 kb (in comparison with
similar genes). My problem is that I only have very little information
(about 100bp) on the sequence of this gene.
Anyone has experience with this ?
Tnx for answering,
Els
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