cycle sequencing

Els Van Geldre Els.VanGeldre at rug.ac.be
Wed Dec 11 11:39:57 EST 1996


Hi,

I'm wondering wether it is possible to perform cycle sequencing reactions 
directly on genomic DNA with a specific primer. I would like to use this 
technique in order to isolate a gene of about 2 kb (in comparison with 
similar genes). My problem is that I only have very little information 
(about 100bp) on the sequence of this gene.
Anyone has experience with this ?
Tnx for answering,

Els 




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