Smeared Bands in PCR

Reid, Thomas M. tar9 at NIOBBS1.EM.CDC.GOV
Wed Dec 11 10:23:02 EST 1996


I am having problems with smeared bands when analyzing PCR products on 
agarose gels.  I am amplifying a 600 bp segment of a 25 Mbp fungal genome. 
 The smearing does not always occur although it seems to be somewhat 
polymerase-specific.  The smearing is above the major band so I don't think 
it's an exonuclease problem.

I would appreciate any advice, thoughts, etc.

Tom Reid



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