Smeared Bands in PCR
Reid, Thomas M.
tar9 at NIOBBS1.EM.CDC.GOV
Wed Dec 11 10:23:02 EST 1996
I am having problems with smeared bands when analyzing PCR products on
agarose gels. I am amplifying a 600 bp segment of a 25 Mbp fungal genome.
The smearing does not always occur although it seems to be somewhat
polymerase-specific. The smearing is above the major band so I don't think
it's an exonuclease problem.
I would appreciate any advice, thoughts, etc.
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