What makes gels smile?

Dr. Duncan Clark duncan at genesys.demon.co.uk
Wed Dec 11 05:52:01 EST 1996

In article <58jqbj$ht0 at falcon.le.ac.uk>, "Dr E. Buxbaum" <EB15 at le.ac.uk>
>brett at BORCIM.WUSTL.EDU (brett) wrote:
>>You tried slow?!? Smiling comes from uneven heat distribution within the >gel, 
>the heat being focused in the center of the gel. This lowers the >resistance in 
>the center, and boosts the current. As the nucleic acids >(and dyes) migrate 
>with this current, they move faster in the center of >the gel. The only solution 
>is to redistribute the heat.
>Yes, that's the way I heard the story. Which just leaves me wondering:
>How come that my gels are always frowning? (And I run them watercooled)

If the electric field does not extend evenly all the way across the gel
then you will get smiling ie electrodes shorter then your gel width.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Dr. Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

More information about the Methods mailing list