What makes gels smile?

Dr. Duncan Clark duncan at genesys.demon.co.uk
Wed Dec 11 05:52:01 EST 1996


In article <58jqbj$ht0 at falcon.le.ac.uk>, "Dr E. Buxbaum" <EB15 at le.ac.uk>
writes
>brett at BORCIM.WUSTL.EDU (brett) wrote:
>>You tried slow?!? Smiling comes from uneven heat distribution within the >gel, 
>the heat being focused in the center of the gel. This lowers the >resistance in 
>the center, and boosts the current. As the nucleic acids >(and dyes) migrate 
>with this current, they move faster in the center of >the gel. The only solution 
>is to redistribute the heat.
>
>Yes, that's the way I heard the story. Which just leaves me wondering:
>How come that my gels are always frowning? (And I run them watercooled)
>

If the electric field does not extend evenly all the way across the gel
then you will get smiling ie electrodes shorter then your gel width.

Duncan 
--------------------------------------------------------------------------------
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Dr. Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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