Questions on the RNA extraction
REGCHAK at USTHK.UST.HK
REGCHAK at USTHK.UST.HK
Wed Dec 11 08:12:33 EST 1996
Hi all,
I did a total RNA extraction on plant tissue by using the Guanidinium
Method as usual. However, when I finished all the preciptation and washing
step, I cann't dissolve all my suspected RNA pellet in the 400ul DEPC
treated water even under 65oC for half an hour. I am sure that 170mg leaves
should not cause such yield of RNA. What's wrong in my RNA pellet? And I
don't whether too dry of the pellet will cause the loss of the yield?
Thanks you for any suggested answer.
Regina
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