Linear PCR

Keith Grimaldi regokag at ucl.ac.uk
Wed Dec 11 05:44:22 EST 1996


Linear amplification could happen if you have an excess of DNA 
template (easy to do when using plasmid templates). 

Other possibilities are not enough of some component such as 
primers, dNTP's , enzyme, etc. Or reaction not efficient due to 
buffer compostion - try Mg titration.

Or you maybe doing too many cycles and are just looking at the 
linear part which happens after the exponential phase but before the 
plateau.

keith grimaldi
oncology
ucl

Ed Wang wrote:
> 
> Can anybody think of a reason that a PCR reaction would be linear rather
> than exponential (other than only one primer is present)?
> 
> Thanks in advance...
> Ed Wang




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