regokag at ucl.ac.uk
Wed Dec 11 05:44:22 EST 1996
Linear amplification could happen if you have an excess of DNA
template (easy to do when using plasmid templates).
Other possibilities are not enough of some component such as
primers, dNTP's , enzyme, etc. Or reaction not efficient due to
buffer compostion - try Mg titration.
Or you maybe doing too many cycles and are just looking at the
linear part which happens after the exponential phase but before the
Ed Wang wrote:
> Can anybody think of a reason that a PCR reaction would be linear rather
> than exponential (other than only one primer is present)?
> Thanks in advance...
> Ed Wang
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