Using heterogenous genomic DNA fragments as probes in Southern Blots

patricia ohana pohana at
Wed Dec 11 04:55:14 EST 1996

I have two questions.

1)I am trying to radiolabel genomic DNA fragments (heterogenous pool).
Using a standard oligolabel kit using random primers. Does anyone have 
experience doing such a labeling, and how do you suggest optimizing the reaction conditions.

2) Does anyone have experience with long PCR using some of the new Taq Polymerases that 
recently have become commercially available. I have a lambda gt11 clone whose insert is almost
impossible to visualize/identify, possibly due to loss of one of the EcoRI cloning sites.
My idea is to use the lambda gt11 universal primers, and my clone as the template.
Patricia Ohana PhD.

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