Amplyfing cDNA libraries

[Ian A. York]

In article <19961211231200.SAA09064 at ladder01.news.aol.com>,
Robmit <robmit at aol.com> wrote:
>
>I have a plasmid cDNA library that I need to Amplify.  Is there any good
>way of doing this other than plating out 100 150mm plates?  Can I grow it
>up in liquid?

I think that plates are generally accepted to be the best way of keeping
the proportions of your inserts reasonably accurate - otherwise you end up
with more of a problem with overgrowth of some inserts at the expense of
others.

I don't think you need anywhere near 100 plates, though, since you can
plate 50,000 colonies + per plate without getting them too crowded; if you
want around a million colonies, then 20 plates should do you.

Ian
-- 
      Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
      "-but as he was a York, I am rather inclined to suppose him a
       very respectable Man." -Jane Austen, The History of England