I'm sorry
Chang,jihun
j6544 at unitel.co.kr
Fri Dec 13 01:42:56 EST 1996
Chang,jihun wrote:
--
Dear bionetter.
Thank you for your reply.
Your kind advices moved me.
Thank you very much for your reply.
But I made big mistake.
Oh! my GOD!!!
I misspelled.
I'm trying to ligate not 'Hind III'-digested genomic DNA ,but 'HaeIII'.
I'm very sorry for my mistake.
Please, show me your kindness once more.
Good-bye .
P.S.
Condition of my competent cell(DH5a) is not so bad.
In 82mm plate, 200-300 blue colonies & 15-20 white colonies.
I did not treat CIP into vector.
I digested genomic DNA with HaeIII overnight, and second boosting 4-5hrs
next day.
More information about the Methods
mailing list