j6544 at unitel.co.kr
Fri Dec 13 01:42:56 EST 1996
Thank you for your reply.
Your kind advices moved me.
Thank you very much for your reply.
But I made big mistake.
Oh! my GOD!!!
I'm trying to ligate not 'Hind III'-digested genomic DNA ,but 'HaeIII'.
I'm very sorry for my mistake.
Please, show me your kindness once more.
Condition of my competent cell(DH5a) is not so bad.
In 82mm plate, 200-300 blue colonies & 15-20 white colonies.
I did not treat CIP into vector.
I digested genomic DNA with HaeIII overnight, and second boosting 4-5hrs
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