cloning problems

thees at mzdmza.zdv.uni-mainz.de thees at mzdmza.zdv.uni-mainz.de
Mon Dec 9 04:03:33 EST 1996


Hi,
this is Rudi from Mainz/Germany. I have a problem with cloning a cDNA
clone into a pGST Vektor. Because of different pGST- and "cDNA clone
Vektor"- polylinker it is only possible to ligate the clone with EcoRI; a
site directed ligation is not possible. My problem is now that all of my
inserts are in the false direction. So is there anyone with the same
ligation problem or does anybody knows a solution to this problem? 

email  thees at mzdmza.zdv.uni-mainz.de



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