Mitochondrial DNA cloning

jmorrow at bio.usyd.edu.au jmorrow at bio.usyd.edu.au
Thu Dec 12 20:28:00 EST 1996


I wish to clone a 1.5kb PCR fragment of the mitochondrial A+T rich region
using the TA cloning technique.  I have tried ligating it into the vectors
pGem-T and pBluescript, and into cells DH5alpha, JM109 and XL1-blue.  The
very few inserts I retrieve are all way too small. Can anyone please help
me with this?

Jennifer Morrow



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